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Porovnání účinnosti přímé a nepřímé metody genetické transformace u bramboru (Solanum tuberosum L.) / A comparison of efficacy of direct and indirect methods of genetic transformation of potato (Solanum tuberosum L.)

Potato is one of the main targets for genetic improvement by gene transfer. The aim of this study was to compare the efficacy of genetic transformation of potato, cultivar Bintje, using two methods: Agrobacterium tumefaciens mediated transformation and microprojectile bombardment. The same plasmid p35SGUSint, which cosists of 35S CaMV promoter, gus and nptII genes, was used for both transformations of internodal potato explants. Kamamycin selection, transient and stable expressions of {$\beta$}-glucuronidase and PCR amplification of gus and nptII transgenes were used for transgenic plant selection, identification and analysis.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:45855
Date January 2008
CreatorsPŘIBYLOVÁ, Marie
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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