The aim tis work was study of diversity in carrot (Daucus carota), the analysis of the homogeneity of the parent material, the evaluation of the success of the crossing, and the resolution of the sterile and fertile genotypes of the samples used in crossing. Four hybrid carrot varieties and 12 lines were analyzed, four of which were sterile (CMS), with ten SSR markers and six CMS markers. Based on the calculated statistical values of DI, PI and PIC, the markers were highly variable. A total of 45 alleles were detected, with a mean of 4.5 alleles per locus and 4 zero alleles. The size of the allele ranged between 180 and 380 bp. The success rate of the crossing was evaluated, when the crossing of the Koloseum variety was evaluated as the most successful. Four SSR markers have been recommended as suitable for further study of the diversity and success rate of carrot crosses, namely GSSR-6, GSSR-16, GSSR-91 and GSSR-9. CMS markers successfully identified the sterile and fertile lines at one crossing (variety Koloseum) by Atp8 marker.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427137 |
| Date | January 2018 |
| Creators | Sejbalová, Darina |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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