In the 10-mer RNA duplex model system a 4-isocyano TEMPO spin-label is individually attached to one strand and two strands are annealed to measure distances. This methodology is limited to systems in which two oligonucleotides are annealed together. To circumvent this limitation and also to explore single-strand dynamics a new methodology was implemented, double spin-labeling. Double spin-labeled single-stranded RNA was investigated as a single-strand and within a duplex via MALDI-TOF-MS, EPR spectroscopy and RP-HPLC. A double spin-labeling strategy in this work will be applicable to large complex RNAs like Group I intron of Tetrahymena thermophilia. Captain Daniel Lawrence, was one of four sloops detailed to serve as a transport to
supply the British Florida garrisons. The Industry ran aground on the bar outside of
St. Augustine's harbour on May 6, 1764. The transport was carrying six-pound
cannons, ammunition and artificer's tools.
Further investigation of documents describing eighteenth-century trade and
shipping to St. Augustine led to the discovery that the Lawrence family of sea
captains provided a vital link between British New York and Spanish St. Augustine.
An examination of the materials recovered from Site 8SJ3478 sheds light on exactly
what a particular vessel carried during a period of transition in Florida's history.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/3304 |
| Date | 12 April 2006 |
| Creators | Franklin, Marianne |
| Contributors | Hamilton, D.L. |
| Publisher | Texas A&M University |
| Source Sets | Texas A and M University |
| Language | en_US |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | 46251154 bytes, 41976534 bytes, electronic, application/pdf, application/pdf, born digital |
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