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Loss of chromosome 6q is frequently seen in gastric carcinoma of all stages.

Li Chung Yi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 110-123). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iv / TABLE OF CONTENTS --- p.v / LIST OF FIGURES --- p.ix / LIST OF TABLES --- p.xi / Chapter I. --- INTRODUCTION --- p.1 / Chapter I.1 --- Gastric Carcinoma --- p.1 / Chapter I.2 --- Etiology of Gastric Carcinoma --- p.2 / Chapter I.2.1 --- Environmental Factors: --- p.2 / Chapter I.2.2 --- Helicobacter Pylori Infection: --- p.2 / Chapter I.2.3 --- Genetic Factors: --- p.3 / Chapter I.2.4 --- Other Factors: --- p.4 / Chapter I.3 --- The Lauren Classification of Gastric carcinoma --- p.5 / Chapter I.3.1 --- Histolopathology of Intestinal Type of Gastric Carcinoma --- p.5 / Chapter I.3.2 --- Histopathology of Diffuse Type of Gastric Carcinoma --- p.8 / Chapter I.4 --- Cytogenetics Studies in Gastric Carcinoma --- p.10 / Chapter I.4.1 --- Cytogenetic Studies of Gastric carcinoma --- p.10 / Chapter I.5 --- Molecular Studies of Gastric Carcinoma --- p.14 / Chapter I.5.1 --- Genetic Instability --- p.14 / Chapter I.5.2 --- Amplification/ Mutation of Oncogenes --- p.15 / Chapter I.5.3 --- Alterations of Tumor Suppressor Genes --- p.20 / Chapter I.5.4 --- Cell Adhesion Molecules --- p.23 / Chapter I.5.5 --- Molecular Studies on Intestinal Metaplasia --- p.27 / Chapter II. --- LONG ARM OF CHROMOSOME 6 --- p.28 / Chapter III. --- BRIEF REVIEWS OF THE TECHNIQUES USED IN THIS STUDY --- p.34 / Chapter III.1 --- Comparative Genomic Hybridization (CGH) --- p.34 / Chapter III.2 --- Loss of Heterozygosity (LOH) --- p.37 / Chapter III.3 --- Methylation-Specific PCR (MSP) --- p.38 / Chapter IV. --- OBJECTIVES OF STUDY --- p.40 / Chapter V. --- MATERIALS AND METHODS --- p.41 / Chapter V.l --- Sample Collections --- p.41 / Chapter V.1.1 --- Patients Information for CGH Studies --- p.42 / Chapter V. 1.2 --- Patients Information for LOH Studies --- p.42 / Chapter V.2 --- Extraction of Genomic DNA for Tumor and Normal Tissues --- p.47 / Chapter V.2.1 --- Extraction of Genomic DNA from Frozen Tissues or Paraffin Embedded Sections --- p.47 / Chapter V.2.2 --- Extraction of Genomic DNA from Blood --- p.48 / Chapter V.3 --- Comparative Genomic Hybridization (CGH) of Gastric Carcinoma --- p.49 / Chapter V.3.1 --- Preparation of Normal Metaphase Slides --- p.49 / Chapter V.3.2 --- Metaphase Slide Denaturation --- p.49 / Chapter V.3.3 --- Nick Translation for DNA Labeling --- p.50 / Chapter V.3.4 --- Dot Blot Assay for Biotin and Digoxigenin-Labeled DNA --- p.51 / Chapter V.3.5 --- "Probe Preparation, Denaturation and Hybridization" --- p.51 / Chapter V.3.6 --- Post hybridization Washing and Detection --- p.52 / Chapter V.3.7 --- Image Acquisition and Analysis of CGH Images --- p.53 / Chapter V.4 --- Loss of Heterozygosity (LOH) Analysis on Chromosome 6q --- p.55 / Chapter V.4.1 --- Microsatellite Markers --- p.55 / Chapter V.4.2 --- Polymerase Chain Reaction (PCR) --- p.57 / Chapter V.4.3 --- Denaturing Polyacrylamide Gel Electrophoresis --- p.58 / Chapter V.4.4 --- SYBR Gold Nucleic Acid Gel Staining and Image Viewing --- p.58 / Chapter V.4.5 --- Assessment of Loss of Heterozygosity (LOH) --- p.59 / Chapter V.4.6 --- Statistical Analysis --- p.61 / Chapter V.5 --- Methylation Specific Polymerase Chain Reaction (MSP) --- p.62 / Chapter V.5.1 --- Bisulfite Modification of DNA --- p.62 / Chapter V.5.2 --- Mehtylation Specific PCR --- p.63 / Chapter VI. --- RESULTS --- p.66 / Chapter VI.1 --- Results of CGH --- p.66 / Chapter VI. 1.1 --- Chromosomal Copy Number Aberrations in Gastric Carcinoma --- p.66 / Chapter VI. 1.2 --- Comparison of CGH Results with Intestinal and Diffuse Type of Gastric Carcinoma --- p.67 / Chapter VI.2 --- LOH Analysis of Chromosome 6q --- p.73 / Chapter VII. --- DISCUSSIONS --- p.83 / Chapter VII.l --- Discussions on CGH --- p.83 / Chapter VII.2 --- Discussions on LOH Study --- p.89 / Chapter VII.2.1 --- Two Distinct Deletion Regions --- p.89 / Chapter VII.2.2 --- Possible Candidate Suppressor Genes in Two Deletion Regions --- p.93 / Chapter VII.2.3 --- Infrequent Loss of IGF2R Gene --- p.95 / Chapter VII.3 --- Relationship Between Intestinal Metaplasia and Gastric Carcinoma --- p.99 / Chapter VII.4 --- Microsatellite Instability --- p.100 / Chapter VII.5 --- Correlations --- p.103 / Chapter VII.6 --- Comparison Between CGH and LOH Results on Chromosome 6 --- p.104 / Chapter VII.7 --- Conclusions --- p.106 / Chapter VII.8 --- Limitations of the Study --- p.107 / Chapter VII.8.1 --- Limitation of CGH --- p.107 / Chapter VII.8.2 --- Limited Information Supply by LOH Analysis --- p.107 / Chapter VII.8.3 --- Small Sample Size --- p.107 / Chapter VII.9 --- Future Studies --- p.108 / Chapter VIII. --- REFERENCES --- p.110

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323652
Date January 2001
ContributorsLi, Chung Yi., Chinese University of Hong Kong Graduate School. Division of Anatomical and Cellular Pathology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 123 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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