Hyperglycemia is associated with a higher risk for the development of cardiovascular diseases such as atherosclerosis and hypertension. Hyperglycemia-induced generation of reactive oxygen species and the endothelial dysfunction largely account for this phenomenon. Ergothioneine is a naturally occurring amino acid that is abundantly found in mushroom. Numerous benefits have been found associated with ergothioneine such as cation chelating, regulation of gene expression, improvement in immunity and bioenergetics, and of most concern its antioxidative property.
The aim of this study was to investigate whether mushroom extract and synthetic ergothioneine can exert protective effect on endothelial cells against oxidative stress. Human umbilical vein endothelial cells served as the cell model. Pyrogallol, hydrogen peroxide and high glucose were used to create the oxidative stress condition in endothelial cells. Biochemical assay was used to measure the viability of the cells. It was found that only the mushroom extract could significantly reduce the cell death induced by pyrogallol. Both the mushroom extract and synthetic ergothioneine significantly decreased the cell death induced by high glucose. However, neither mushroom extract nor synthetic ergothioneine have any positive effect on hydrogen peroxide-induced cell death.
These results indicated that mushroom extract and synthetic ergothioneine did exert certain level of protective effect on endothelial cells. However, this protective effect is relatively weak. Besides, it is still unclear if antioxidation is the sole mechanism accounting for the cytoprotective effect of ergothioneine. Further investigation is required to examine if other mechanisms are also involved. / published_or_final_version / Pharmacology and Pharmacy / Master / Master of Medical Sciences
| Identifer | oai:union.ndltd.org:HKU/oai:hub.hku.hk:10722/173887 |
| Date | January 2012 |
| Creators | Zhou, Tianjiao., 周天骄. |
| Publisher | The University of Hong Kong (Pokfulam, Hong Kong) |
| Source Sets | Hong Kong University Theses |
| Language | English |
| Detected Language | English |
| Type | PG_Thesis |
| Source | http://hub.hku.hk/bib/B48334455 |
| Rights | The author retains all proprietary rights, (such as patent rights) and the right to use in future works., Creative Commons: Attribution 3.0 Hong Kong License |
| Relation | HKU Theses Online (HKUTO) |
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