VAMP-7 has been found to interact with SNAP-23, a t-SNARE that functions in relocating
granule membranes in response to stimulation, and plays a large role in the regulation of granule
release from mast cells in response to an allergic reaction. While evidence suggests that VAMP-7 is
active in antibody release in the innate immune system, little investigation has been completed on
VAMP-7 interaction in specific antibody release of B lymphocytes of the humoral immune system.
Little research has previously focused on vesicular transport within B lymphocytes, leaving
molecular mechanisms within B lymphocytes a mystery. Immunodot blots, western blots, and
immunoflourescent microscopy were all utilized with the goal of identifying the presence of VAMP-
7. Immunobot blots for both 55D1 and 240E cells were all negative for the presence of VAMP-7.
However, VAMP-7 was detected using immunoflourescent microscopy in both 55D1 and 240E cell
lines. / Access to thesis permanently restricted to Ball State community. / Department of Physiology and Health Science
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:123456789/198122 |
| Date | 03 May 2014 |
| Creators | French, Kyleigh Anne |
| Contributors | Kelly-Worden, Marie L. |
| Source Sets | Ball State University |
| Detected Language | English |
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