Made available in DSpace on 2014-12-17T14:05:20Z (GMT). No. of bitstreams: 1
VivianeKSM_TESE.pdf: 2717769 bytes, checksum: dc3d156a1c1b3c1791c93a0e2266556f (MD5)
Previous issue date: 2011-12-13 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Chromobacterium violaceum is a free-living bacillus, Gram-negative commonly
found in water and sand of tropical and subtropical regions. One of its main
characteristic it's the ability to produce the purple pigment named violacein, that
shows countless biological activities. In 2003, the genome of this organism was
totally sequenced and revealed important informations about the physiology of
this bacteria. However, few post-genomics studies had been accomplished.
This work evaluated the protein profile of C. violaceum cultivated in LB medium
at 28?C that allowed the identification and characterization of proteins related to
a possible secretion system that wasn't identified and characterized yet in C.
violaceum, to the quorum sensing system, to regulatory process of transcription
and translation, stress adaptation and biotechnological potential. Moreover, the
response of the bacteria to UVC radiation was evaluated. The comparison of
the protein profile, analyzed through 2-D electrophoresis, of the control group
versus the treatment group allowed the identification of 52 proteins that arose
after stress induction. The obtained results enable the elaboration of a stress
response pathway in C. violaceum generated by the UVC light. This pathway,
that seems to be a general stress response, involves the expression of proteins
related to cellular division, purine and pirimidine metabolism, heat chock or
chaperones, energy supply, regulation of biofilm formation, transport, regulation
of lytic cycle of bacteriophages, besides proteins that show undefined function.
Despite the response present similarities with the classic SOS response of E.
coli, we still cannot assert that C. violaceum shows a SOS-like response, mainly
due to the absence of characterization of a LexA-like protein in this organism / Chromobacterium violaceum ? um bacilo de vida-livre, Gram-negativo
comumente encontrado no solo e nas ?guas de regi?es tropicais e subtropicais.
Uma das principais caracter?sticas deste organismo ? sua capacidade de
produzir o pigmento violace?na, o qual apresenta in?meras atividades
biol?gicas. Em 2003, o genoma deste organismo foi completamente
sequenciado e revelou informa??es importantes sobre a fisiologia desta
bact?ria. Por?m, poucos estudos p?s-gen?micos tem sido realizados. Este
trabalho avaliou o perfil proteico de C. violaceum cultivada em meio LB a 28?C,
o que permitiu a identifica??o de prote?nas relacionadas a um poss?vel sistema
de secre??o ainda n?o identificado e caracterizado em C. violaceum, ao
sistema quorum sensing, a processos regulat?rios da transcri??o e tradu??o,
adapta??o ao estresse e ao potencial biotecnol?gico. Al?m disso, a resposta
desta bact?ria ? radia??o UVC foi avaliada. A compara??o do perfil prot?ico,
analisado por eletroforese 2-D, do controle versus tratado possibilitou a
identifica??o de 52 prote?nas que surgiram ap?s a indu??o do estresse. Os
resultados obtidos permitiram a elabora??o de uma via de resposta de C.
violaceum ao estresse gerado pela luz UVC. Esta via, que parece ser de
resposta geral ao estresse, envolve a express?o de prote?nas relacionada ?
divis?o celular, metabolismo de purinas e pirimidinas, choque t?rmico ou
chaperonas, fornecimento de energia, regula??o da forma??o de biofilme,
transporte, regula??o do ciclo l?tico de bacteri?fagos, al?m de prote?nas que
ainda n?o apresentam fun??o caracterizada. Apesar da reposta apresentar
similaridades com a SOS cl?ssica de E. coli, ainda n?o podemos afirmar que C.
violaceum apresenta uma resposta SOS-like, principalmente devido a aus?ncia
da caracteriza??o de um prote?na LexA-like neste organismo
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12642 |
| Date | 13 December 2011 |
| Creators | Medeiros, Viviane Katielly Silva |
| Contributors | CPF:32398336468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781004Y8, Lima, Lucymara Fassarela Agnez, CPF:00297997750, http://lattes.cnpq.br/1083882171718362, Lima, Jo?o Paulo Matos Santos, CPF:79332021368, http://lattes.cnpq.br/3289758851760692, Pirovani, Carlos Priminho, CPF:00171845765, http://lattes.cnpq.br/9615448923708075, Grangeiro, Thalles Barbosa, CPF:31160166315, http://lattes.cnpq.br/3114375474778667, Medeiros, S?lvia Regina Batistuzzo de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Biotecnologia, UFRN, BR, Biotecnologia Industrial; Biotecnologia em Agropecu?ria; Biotecnologia em Recursos Naturais; Biotecn |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/embargoedAccess |
Page generated in 0.0023 seconds