Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity

Description

No / TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a
mono-ADP-
ribosyltransferase
that has emerged as an important regulator of innate immunity, stem cell
pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying
its target proteins are critical to understanding its cellular function. Here we describe methods that
we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.

Links & Downloads

1. Hutin D, Grimaldi G and Matthews J (2018) Methods to study TCDD-inducible poly-ADP-ribose polymerase (TIPARP) mono-ADP-ribosyltransferase activity. In: Chang P (ed) ADP-ribosylation and NAD+ utilizing enzymes. Methods in Molecular Biology: 1813. Humana: New York.
2. http://hdl.handle.net/10454/18336
3. https://doi.org/10.1007/978-1-4939-8588-3_8

Tags

Immunoprecipitation

Protein purification

Biotinylated-NAD+

32P-NAD+

Additional Fields

Identifer	oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/18336
Date	11 August 2018
Creators	Hutin, D., Grimaldi, Giulia, Matthews, J.
Source Sets	Bradford Scholars
Language	English
Detected Language	English
Type	Book chapter, No full-text in the repository