I december 2019 rapporterades fall av oidentifierad lunginflammation, senare känd som Covid-19. Antalet fall ökar fortfarande snabbt i världen och därmed utgör Covid-19 ett extraordinärt hot mot folkhälsan. Studier har visat en ökad mängd proinflammatoriska cytokiner TNF-α, associerade med andningssvårigheter samt en ökad kortisolproduktion. Utsöndring av kortisol och TNF-α är associerade med fysiska- och psykiska hälsoeffekter, sjukdomsgrad samt dödsfall av Covid-19. Syftet med studien var att undersöka utsöndringen av biomarkörerna kortisol och TNF-α i saliv hos individer tidigare diagnostiserade med Covid-19 samt hos individer utan diagnostiserad Covid-19 (kontrollgrupp). Det för att undersöka huruvida kvarvarande symtom kunde associeras till biomarkörerna. Studien inkluderade 40 individer, varav 20 individer tidigare diagnostiserade med Covid-19 samt 20 individer utan diagnostiserad Covid-19. Saliv och ett frågeformulär om deras allmänna hälsotillstånd och symtom kopplade till Covid-19 infektion samlades in och analyserades. Salivprover tagna på morgon analyserades med ELISA och resultatet jämfördes mellan grupperna. Inga signifikanta skillnader visade mellan grupperna gällande biomarkörer. Resultaten visade dessutom inga korrelation mellan symtom och biomarkörer samt att inga förhöjda nivåer av analyserade biomarkörer kan associeras med kvarvarande symtom efter en Covid-19 infektion. / A cluster of unidentified pneumonia cases, later known as Covid-19, were reported in December 2019. The number of Covid-19 cases is still rapidly increasing and poses an extraordinary threat to our public health. Studies have shown an increased level of the cytokine TNF-α, associated with severe respiratory symptoms as well as an increased cortisol production. Together associated with physical and mental health effects, disease severity and increased mortality of Covid-19. This study aimed to evaluate the secretion of cortisol and TNF-α in the saliva of individuals previously diagnosed with Covid-19 and individuals not diagnosed with Covid-19 (control group). This to examine whether the residual symptoms can be associated with the analyzed biomarkers. The study included 40 participants, 20 individuals diagnosed with Covid-19 and 20 individuals not diagnosed with Covid -19. Saliva and a questionnaire concerning their overall health and possible symptoms associated with Covid-19 infection were collected and analyzed. Saliva morning samples were analyzed with ELISA and results were compared between the groups. No significant differences were shown between the groups regarding the biomarkers. Also, the results showed no correlations between symptoms and biomarkers, nor elevated levels of analyzed biomarkers could be associated with residual symptoms after a Covid-19 infection.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:hj-52980 |
| Date | January 2021 |
| Creators | Poolsri, Louise |
| Publisher | Jönköping University, HHJ, Avd. för naturvetenskap och biomedicin, Jönköping University, HHJ. Biomedicinsk plattform |
| Source Sets | DiVA Archive at Upsalla University |
| Language | Swedish |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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