The target of this thesis was to obtain a sufficient number of DNA samples from individuals Breed Large Münsterländer and they perform genotyping newly described mutation c.555> G in exon 2 of the gene TYRP1. In the case of this mutation in the breed we studied, another aim of the work was to propose breeding measures that would limit the spread of this undesirable mutation. At the beginning of the literary overview, the breed of the great Münsterlander, his history, the breed, the standard with an emphasis on the permitted coloring and the current breeding of a large Mässlandlander in the Czech Republic. The thesis describes the individual genetic foundations of coloring and explains the principles of the methods used in their own work. In its own work genotyping was performed on the mutation in exon 2 of the TYRP1 gene by polymerase chain reaction and sequencing methods. This mutation was not demonstrated in either of the 113 tested individuals of the Greater Münsterlander. It was also used of the available commercial tests previously described mutations in the gene TYRP1 on which were worked out plans for auxiliary mating as an aid to farmers to avoid the herd to increase the number of recessive homozygotes undesirable brown coat color or heterozygotes, who carry a mutated allele.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:376191 |
| Date | January 2018 |
| Creators | FRYŠOVÁ, Adéla |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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