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Previous issue date: 2007-12-05 / Radiobiocomplexes are used to obtain images in nuclear medicine and employed in basic research. Blood constituents labeled with technetium-99m (99mTc) have also been employed as radiobiocomplexes and used also experimental model for evaluation of the biological effects of natural or synthetic drugs. The analysis of the morphology and the morphometrics parameters (perimeter/?rea ratio) can be used to evaluate the effects of drugs upon the structure of the membrane of red blood cells. Cinnamomum zeylanicum (cinnamon) is a spice used as herbal medicine to treat diseases. The aim of this study was to evaluate the effect of in vitro and in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. In the in vitro treatment, isolated blood sample from animals were incubated with cinnamon extract. In the in vivo treatment, blood samples were also withdrawn from animals treated with cinnamon extract. In both cases, the radiolabeling of blood constituents was done. The morphological analysis of red blood cells was also done. As control, blood or animals treated with NaCl 0.9%. The data obtained on the labeling of blood constituents with 99mTc experiments indicated that the in vitro treatment with cinnamon extract was capable to decrease signiicantly (p<0.05) the percentage of radioactivity in cellular compartments and on the fixation of cellular and plasma proteins. These effects were not observed on the in vivo treatment. The results obtained for the morphology of red blood cells suggest that the in vitro and in vivo treatments did not alter the morphology and the perimeter/area ratio. The in vitro treatment with aqueous cinnamon extract could affect the membrane structures related with the oxidation status of the stannous ion pertechnetate ion, altering the labeling of blood constituentes with 99mTc. This study was a multidisciplinary experimental research. It was developed with the contribution of the different Departments and Services of the Hospital Universit?rio Pedro Ernesto of the Universidade do Estado do Rio de Janeiro / Radiobiocomplexos s?o utilizados para obten??o de imagens em medicina nuclear e empregados na pesquisa b?sica. Constituintes sang??neos marcados com tecn?cio-99m t?m sido empregados como radiobiocomplexos e utilizados como modelo experimental para avalia??o de efeitos biol?gicos de drogas naturais ou sint?ticas. A an?lise da forma e de par?metros morfom?tricos (rela??o per?metro/?rea) pode ser usada para avaliar os efeitos de drogas sobre a estrutura da membrana de hem?cias. Cinnamomum zeylanicum (canela) ? uma especiaria usada como planta medicinal para tratar doen?as. O objetivo desse estudo foi avaliar efeitos de um extrato aquoso de Cinnamomum zeylanicum na marca??o de constituintes sangu?neos com tecn?cio-99m e na morfologia de hem?cias de ratos Wistar. No tratamento in vitro, amostras de sangue de animais foram isoladas e incubadas com um extrato de canela. No tratamento in vivo, animais foram tratados com um extrato de canela e amostras de sangue foram isoladas. Em ambas as situa??es foram realizadas a radiomarca??o de constituintes sangu?neos e a an?lise morfol?gica. Como controle, o sangue ou os animais foram tratados com cloreto de s?dio 0,9%. Os dados obtidos nos experimentos de marca??o de constituintes sang??neos com tecn?cio-99m indicaram que o tratamento in vitro com extrato de canela foi capaz de diminuir significativamente (p<0,05) a porcentagem de radioatividade nos compartimentos celulares e na fixa??o das prote?nas celulares e plasm?ticas. Esses efeitos n?o foram observados no tratamento in vivo. Os resultados obtidos na morfologia de hem?cias sugerem que os tratamentos in vitro e in vivo n?o alteram a forma nem a raz?o per?metro/?rea. O tratamento in vitro com o extrato aquoso de canela poderia afetar estruturas da membrana celular relacionadas ao transporte de ?ons ou ao estado de oxida??o do ?on estanoso ou do ?on pertecnetato, alterando a marca??o de constituintes sang??neos com tecn?cio-99m. Esse estudo ? uma pesquisa experimental com car?ter multidisciplinar. Foi desenvolvido em colabora??o com diferentes Departamentos e Servi?os da ?rea biom?dica do Hospital Universit?rio Pedro Ernesto, UERJ, atestando o car?ter multidisciplinar da pesquisa
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13172 |
| Date | 05 December 2007 |
| Creators | Benarroz, M?nica de Oliveira |
| Contributors | CPF:33810990787, http://lattes.cnpq.br/9941440001544010, Palestro, Christopher J., Medeiros, Aldo da Cunha, CPF:07117701404, http://lattes.cnpq.br/9873289951810864, Bernardo Filho, M?rio |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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