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Temperature-Sensitive Mutants of Bacteriophage PBS 2

<p> Temperature-sensitive mutants of the bacteriophage PBS 2 were isolated from lysates treated with various mutagens. Complementation tests assigned the mutants to 10 cistrons. The mutants were mapped by two factor crosses and formed a linear map approximately 50 recombination percent in length. </p> <p> A method for phage transformation was developed. By the use of wild type DNA fragments fractionated according to their guanine + cytosine content in HgCs₂SO₄ gradients, it was possible to determine the base composition of certain regions of the chromosomes. </p> / Thesis / Doctor of Philosophy (PhD)

Identiferoai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/17968
Date11 1900
CreatorsHerrington, Muriel Bella
ContributorsTakahashi, I., Biology
Source SetsMcMaster University
LanguageEnglish
Detected LanguageEnglish

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