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Transcriptome profiling of two Arabidopsis Farnesyl diphosphate synthase mutants for understanding terpenoids metabolism.

Yu, Pui Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 68-78). / Abstracts in English and Chinese. / Acknowledgements --- p.iv / Abstract --- p.v / Table of Contents --- p.ix / List of Figures --- p.xii / List of Tables --- p.xiv / List of Abbreviations --- p.xv / Chapter Chapter 1. --- General Introduction --- p.1 / Chapter Chapter 2. --- Literature Review --- p.5 / Chapter 2.1 --- The importance of terpenoids --- p.5 / Chapter 2.2 --- The difficulties in synthesizing terpenoids --- p.8 / Chapter 2.3 --- Structure and classification of terpenoids --- p.9 / Chapter 2.4 --- MVA and MEP pathways of terpenoid biosynthesis in higher plants --- p.12 / Chapter 2.4.1 --- The MVA pathway --- p.16 / Chapter 2.4.2 --- The MEP pathway --- p.18 / Chapter 2.5 --- The crosstalk between MVA and MEP routes --- p.20 / Chapter 2.6 --- The farnesyl diphosphate is a key enzyme in terpenoid biosynthetic pathway --- p.20 / Chapter 2.7 --- The glutaredoxin system --- p.22 / Chapter Chapter 3. --- Materials and Methods --- p.25 / Chapter 3.1 --- Plant materials and growth condition --- p.25 / Chapter 3.2 --- DNA extraction and screening of fps mutants --- p.25 / Chapter 3.3 --- Validation of the fps mutant by semi-quantitative RT-PCR --- p.26 / Chapter 3.4 --- Semi-quantitative RT-PCR analysis of the fps mutants --- p.28 / Chapter 3.5 --- Genechip analysis of fps mutants --- p.29 / Chapter 3.6 --- Enzyme assays --- p.29 / Chapter 3.7 --- Triterpene and sterol analysis of fps mutants --- p.30 / Chapter 3.8 --- Preparation of carotenoid standards for carotenoid analysis --- p.31 / Chapter 3.9 --- Carotenoids analysis of fps mutants by HPLC --- p.31 / Chapter 3.10 --- Subcellular localization of FPS 1 and FPS2 by transient expression --- p.33 / Chapter Chapter 4. --- Results --- p.36 / Chapter 4.1 --- Screening of fpsl and fps2 homozygous mutants --- p.36 / Chapter 4.2 --- Validation of fps mutants by RT-PCR and enzyme activity assay --- p.36 / Chapter 4.3 --- Genechip analysis of two fps mutants --- p.40 / Chapter 4.3.1 --- Quality control and normalization of microarray sample --- p.40 / Chapter 4.3.2 --- Normalization and identification of differentially expressed genes --- p.42 / Chapter 4.3.3 --- GO annotation of differentially expressed genes in fps mutants --- p.43 / Chapter 4.3.4 --- Genes participate in stress and defense response were differentially expressed in both fpsl and fps2 mutants --- p.48 / Chapter 4.3.5 --- Genes in the plastidial pathway were down-regulated --- p.51 / Chapter 4.4 --- Effects of FPS mutations on pathway enzymes --- p.53 / Chapter 4.5 --- Effects of fps mutants on terpenoids and sterol metabolism --- p.55 / Chapter 4.6 --- Comparison on carotenoids and chlorophyll contents --- p.55 / Chapter 4.7 --- Subcellular localization of FPS 1 and FPS2 --- p.61 / Chapter Chapter 5. --- Discussion --- p.62 / Chapter Chapter 6. --- Conclusion --- p.67 / Reference --- p.68 / Appendices --- p.79 / Appendix A. Primers designed for homozygous mutant screening for fps mutants --- p.79 / "Appendix B. Primer pairs designed for fps ORF, common sequence and fpsl specific region" --- p.80 / Appendix C. Primer pairs designed for studying expression level of the downstream genes of FPS --- p.81 / Appendix D. Annotations of differentially expressed genes in fpsl mutant --- p.82 / Appendix E. Annotations of differentially expressed genes in fps2 mutant --- p.84 / Appendix F. Log fold changes of terpenoid pathway genes involved in FPS mutants --- p.94

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326755
Date January 2009
ContributorsYu, Pui Man., Chinese University of Hong Kong Graduate School. Division of Life Sciences.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xvii, 96 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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