The nucleotide sequence of cnjB, a conjugation specific gene of Tetrahymena thermophila, has been determined. The gene encodes a putative polypeptide (CnjB) of 1748 amino acids with a calculated molecular weight of 200 kilodaltons and a pI of 7.9. Four transcription start sites were mapped. The gene has only one genomic copy and is not conserved in yeast (as determined by cross-hybridization experiments with yeast DNA). Analysis of the 13 introns in cnjB, accompanied by 15 other T. thermophila intron sequences, shows that they resemble the nuclear pre-mRNA introns of other eukaryotes. / The carboxy-terminal third of CnjB has three regions with repetitive sequences. One region contains seven retroviral-type zinc fingers and the others contain repeated glycine-rich motifs, a motif seen in the heterogeneous nuclear ribonuclear proteins A1 and A2/B1. Proteins with these motifs have single-strand binding and strand annealing activity. / Synthetic phosphorothioate oligonucleotides antisense to regions of the isoleucyl tRNA synthetase gene transcript did not affect cell growth in a sequence-specific manner when cultures of T. thermophila were grown in their presence.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41772 |
| Date | January 1993 |
| Creators | Taylor, Frances Mary |
| Contributors | Martindale, D. W. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001386304, proquestno: NN94715, Theses scanned by UMI/ProQuest. |
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