The nucleotide sequence of the cnjA cDNA (formerly pC1) from the ciliated protozoan Tetrahymena thermophila was determined. This gene was previously found to be conjugation specific and to peak in expression just prior to or at pachytene in meiotic prophase I. The cnjA message is initiated from four transcription start sites, one minor and three major, and encodes a putative protein (CnjA) of 779 amino acids. The protein has a calculated molecular weight of 89.5 kDa and is mainly hydrophilic with an estimated pI of 9.3. CnjA was found to share no sequence similarities with any known protein to date. The gene's coding region demonstrates an unusual codon choice. Flanking regions of the cnjA genomic locus were amplified by means of the Inverse PCR method but attempts at subcloning and characterizing its promoter region were unsuccessful.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69685 |
| Date | January 1993 |
| Creators | Rosenauer, Angelika |
| Contributors | Martindale, Duane W. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001382975, proquestno: AAIMM91783, Theses scanned by UMI/ProQuest. |
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