Starved cells of Ant-300, a psychrophilic marine Vibrio, maintained
binding and transport systems for glutamic acid, arginine, proline, and
leucine. The uptake of these amino acids was inhibited by the uncoupler
2,4-dinitrophenol indicating that an energized membrane was necessary
for binding and was maintined in starved cells. The levels of macromolecular
synthesis and CO₂ production decreased after the cells had
starved for 24 hours and remained low for 30 days of starvation. Most
of the amino acid taken up by starved cells was held in shock releasable
pools. The substrate affinity of the leucine and arginine binding
proteins increased after 48 hours of starvation while the velocity
decreased. These starvation induced changes remained constant throughout
the 20 day starvation time of the experiment. The maintenance of
binding proteins and an energized membrane by a starved cell which has
no energy input means that the cell is not committed to total inactivity.
The survival form is more of a 'ready and waiting' form, capable of
maintaining itself in an unfavorable environment while retaining the
ability to take advantage of any favorable shift in its surroundings. / Graduation date: 1981
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/30113 |
| Date | 08 August 1980 |
| Creators | Glick, Melissa Ann |
| Contributors | Morita, Richard Y. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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