Tropical Theileriosis is a parasitic disease of calves with a profound economic
impact caused by Theileria annulata, an apicomplexan parasite of the genus
Theileria. Transmitted by Hyalomma ticks, T. annulata infects and transforms
bovine lymphocytes and macrophages into a cancer-like phenotype
characterized by all six hallmarks of cancer. In the current study we investigate
the transcriptional landscape of T. annulata-infected lymphocytes to define genes
and miRNAs regulated by host cell transformation using next generation
sequencing. We also define genes and miRNAs differentially expressed as a
result of the attenuation of a T.annulata-infected macrophage cell line used as a
vaccine. By comparing the transcriptional landscape of one attenuated and two
transformed cell lines we identify four genes that we propose as key factors in
transformation and virulence of the T. annulata host cells. We also identify miR-
126-5p as a key regulator of infected cells proliferation, adhesion, survival and
invasiveness. In addition to the host cell trascriptome we studied T. annulata
transcriptome and identified the role of ROS and TGF-β2 in controlling parasite
gene expression. Moreover, we have used the deep parasite ssRNA-seq data to
refine the available T. annulata annotation. Taken together, this study provides
the full list of host cell’s genes and miRNAs transcriptionally perturbed after
infection with T. annulata and after attenuation and describes genes and miRNAs
never identified before as players in this type of host cell transformation.
Moreover, this study provides the first database for the transcriptome of T.
annulata and its host cells using next generation sequencing.
| Identifer | oai:union.ndltd.org:kaust.edu.sa/oai:repository.kaust.edu.sa:10754/617544 |
| Date | 06 1900 |
| Creators | Rchiad, Zineb |
| Contributors | Pain, Arnab, Biological and Environmental Science and Engineering (BESE) Division, Morrison, Ivan, Langsley, Gordon, Merzaban, Jasmeen, Ghaffour, NorEddine |
| Source Sets | King Abdullah University of Science and Technology |
| Language | English |
| Detected Language | English |
| Type | Dissertation |
| Rights | 2017-07-26, At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-07-26. |
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