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Implementing Fluorescence Lifetime Imaging on a Confocal Microscope

In this thesis, the development and implementation of fluorescence lifetime imaging microscopy that integrates time correlated single photon counting (TCSPC) and a confocal microscope will be described. The TCSPC method has high detection efficiency, with a time resolution limited only by the transit time spread of the detector, and directly delivers the decay functions in the time domain. TCSPC can also be used to obtain images that indicate the fluorescence resonance energy transfer (FRET) effect between critical fluorophores, an important method distinguish the difference between binding and co-localization. Estimation of distances between RET fluorophore pairs can also be established. Additionally, the effects of ion concentration, oxygen concentration, pH value, ..etc. can also be revealed.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0706105-000256
Date06 July 2005
CreatorsChiu, Yi-Chun
ContributorsFu-Jen Kao, Ta-Chau Chang, Chi-Hung Lin, Yueh-Hsin Ping
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706105-000256
Rightscampus_withheld, Copyright information available at source archive

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