Different cryoprotectant media for cryopreservation of embryos has been tested on model species, i.e. common carp (Cyprinus carpio) and common tench (Tinca tinca). The aim of the study was to obtain such cryoprotectants, which will be acceptable for freezing embryos up to the temperature {--}196 oC. Cryoprotectants of 10 % and 20 % methanol or 10 % and 20 % glycerin have been tested on the tench for 21 minutes of incubation on embryos of four stages, meaning at 11, 17, 23 and 29-hrs after activation of gametes. The results showed that the tench embryos were most resistant either to low temperature and or to the application of cryoprotectants in the stage of 29-hrs post gametes activation. On the other hand lower resistances were obtained in the stage of 11-hrs post gamete activation. Embryos of carp 2, 6, 22, 24 and 42-hrs after gametes activation at temperature 18 and 22 oC have been used for testing of concentration series of cryoprotectant methanol and two solutions marked VS1 and VS2 after previous disruption of egg envelope in enzyme alcalaze solution. Results showed linear decreasing resistance of embryos depending on increasing concentration of cryoprotectant methanol. Hatching success even at highest concentration of solution VS1 and VS2 has not declined below 70 %. Achieved results with solution VS2 have been subsequently used for freezing of carp embryos by special methods in cryobiology {--} vitrification. First results showed up to 4 % success of survival after freezing of embryos at {$-$}196 oC.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:46598 |
| Date | January 2007 |
| Creators | ALDORF, Milan |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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