Se analizaron 60 muestras de Salchicha tipo Huacho de los Mercados de Abastos del Cercado de Lima, con la finalidad de determinar la incidencia de Listeria monocytogenes. Estas muestras se llevaron al Laboratorio de Microbiología de la Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, en recipientes estériles, refrigerados y se procesaron dentro de las 24 horas de colectadas.
Para el análisis microbiológico se utilizó la técnica según USDA- FSIS, que consta de tres fases: Pre-enriquecimiento, Enriquecimiento de la muestra y siembra en agares selectivos, pruebas Bioquímicas, Catalasa, Gram positivos y la prueba del CAMP para su confirmación.
La incidencia de L. monocytogenes en Salchicha tipo Huacho, en los Mercados de Abastos del Cercado de Lima es de 78%, siendo un valor considerable y que supera a los valores reportados en estudios a nivel internacional. Estos resultados permiten considerar que la Salchicha tipo Huacho se constituye como un alimento de riesgo potencial para la Salud Pública. / --- 60 samples of the Huacho Sausage from the Food Markets in Cercado de Lima, in Lima, Peru, were analyzed in order to determine the incidence of Listeria monocytogenes.
These samples were taken to the Laboratory of Microbiology to the Faculty of Pharmacy and Biochemistry at the National University of San Marcos. They were taken in sterile containers for being refrigerated. Then they were processed within 24 hours of being collected.
The USDA-FSIS technique was used for microbiological analysis, which consists of three phases: Pre-enrichment of the sample, Sample Enrichment and Reseeding on selective agars. At the same time, some biochemical tests were applied, such as the positive Catalase test, the test of Great Positive, and CAMP test to confirm the results.
The incidence of the Listeria monocytogenes in the Huacho Sausage, from the Food Markets in Cercado de Lima, is 78%. This is quite high, as it exceeds the values reported in international studies.
These results support the conclusion that the Huacho Sausage is a food with a potential risk to the public health.
| Identifer | oai:union.ndltd.org:Cybertesis/oai:cybertesis.unmsm.edu.pe:cybertesis/3388 |
| Date | January 2013 |
| Creators | Pérez Alarcón, María Elizabeth |
| Contributors | Salazar Salvatierra, María Elena |
| Publisher | Universidad Nacional Mayor de San Marcos |
| Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
| Language | Spanish |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Source | Repositorio de Tesis - UNMSM, Universidad Nacional Mayor de San Marcos |
| Rights | info:eu-repo/semantics/openAccess |
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