FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / Um dos principais objetivos do tratamento endodÃntico dos canais radiculares de dentes com polpa necrÃtica consiste em eliminar os microorganismos localizados no sistema de canais radiculares. Dessa forma, esta dissertaÃÃo, constituÃda por um artigo, propÃe-se a avaliar o efeito antibacteriano do preparo quÃmico-mecÃnico e de uma pasta à base de hidrÃxido de cÃlcio sobre bactÃrias presentes em canais radiculares de dentes decÃduos necrosados apÃs trauma bem como verificar a presenÃa dos microorganismos Fusobacterium nucleatum e bacilo pigmentado negro nestes dentes. Seguindo os critÃrios de inclusÃo, a amostra consistiu de 18 dentes, totalizando 14 pacientes. As coletas microbiolÃgicas foram realizadas apÃs a abertura coronÃria (C1) e 72h apÃs a remoÃÃo da medicaÃÃo intracanal (C3), sendo que, para 10 dentes, realizou-se outra coleta apÃs a instrumentaÃÃo (C2). As coletas foram realizadas introduzindo-se sequencialmente 3 cones de papel absorvente estÃril, de diÃmetro visualmente compatÃvel com o do canal radicular, no interior deste por aproximadamente 1 minuto. ApÃs este intervalo, os cones foram removidos, transferidos para um tubo contendo um fluido reduzido para transporte e levados ao laboratÃrio para processamento microbiolÃgico. Na C1, os microorganismos foram isolados em 17/18 (94,4%) dos canais radiculares, sendo a mÃdia de CFUs de 5.4 x 105, na C2, em apenas 1/10 (10%) canal radicular, com uma mÃdia de 4.3 x 102, e na C3 em 15/18 (83.3%), com mÃdia de 1.5 x 105. Houve uma diferenÃa estatisticamente significante entre C1 e C2, o mesmo nÃo ocorrendo entre C1 e C3 e entre C2 e C3. O microorganismo Fusobacterium nucleatum e o bacilo pigmentado negro foram observados em 55.5% (10/18) e 11.1% (2/18), respectivamente, na C1, nÃo sendo detectados na C2, e na C3 estavam presentes em 16.6% (3/18) e 5.5% (1/18), respectivamente. Na C1, observou-se uma predominÃncia de cocos gram-negativos (15/18) e bacilos gram-negativos (14/18), representando 83.3% e 77.8%, respectivamente. Na C2, os Ãnicos morfotipos detectados foram cocos gram-positivos (1/10), presente em 10% das amostras positivas, e na C3, os cocos-gram positivos predominaram (66.7%). Pode-se concluir que o preparo quÃmico-mecÃnico desempenhou sua funÃÃo antibacteriana ao reduzir significativamente o nÃmero de microorganismos do canal principal, porÃm o hidrÃxido de cÃlcio possui um efeito antibacteriano limitado, nÃo sendo capaz de prevenir o recrescimento de bactÃrias apÃs seu uso como medicaÃÃo intracanal. / One of the main objectives of endodontic treatment of roots canals with necrotic pulps consists in eliminating the microorganisms spread throughout the ramifications of root canal system. This study, comprised by one manuscript, had the objective to evaluate the antiseptic efficacy of biomechanical preparation and a calcium hydroxide-paste in root canals of human primary teeth with necrotic pulp after trauma and to detect the microorganisms Fusobacterium nucleatum and black-pigmented bacilli in this teeth. According to stringent inclusion criteria, 18 primary teeth with necrotic pulp were selected. Bacterial samples were taken after crown access (S1) and 72h after the removal of dressing with a calcium hydroxide paste (S3), but to 10 teeth were taken a other bacterial sample after chemomechanical preparation with 0.5% NaOCl as an irrigant (S2). Bacteriological samples were collected by introducing 3 sequential sterile absorbent paper points, of a size visually compatible with the root canal diameter. After approximately 1 min, the paper points were removed and placed in a test tube containing reduced transport fluid and were sent for microbiological evaluation. In the S1, the microorganisms were found in 7/18 (94,4%) of the samples, with a colony forming units (CFUâs) media of 5.4 x 105. In the S2, bacteria were cultured in only 1/10 (10%) root canal, with the CFUâs media of 4.3 x 102, and in the S3 bacteria were cultured in 15/18 (83.3%), with the CFUâs media of 1.5 x 105. A statistically significant reduction in bacterial counts was observed between S1 and S2, however no statistically significant difference was observed for comparisons involving S1 and S3, and S2 and S3. The microorganisms Fusobacterium nucleatum and black-pigmented bacilli were detected in 55.5% (10/18) and 11.1% (2/18), respectively, in the S1, no were found in the S2, and in the S3 were found in 16.6% (3/18) and 5.5% (1/18), respectively. In the S1, the gram-negative cocci (15/18) and gram-negative rods (14/18) were the most prevalent groups (83.3% and 77.8%, respectively). In the S2, the gram-positive cocci was the only group of the bacteria observed (1/10), and in the S3, the gram-positive cocci was the group most commonly recovered (66.7%). It was conclude that biomechanical preparation were important in the antisepsis of the root canal because reduced significantly the number of bacteria in the main canal however the calcium hydroxide paste had a antibacterial efficacy limited, no prevent the regrowing bacterial after used as dressing intracanal.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.teses.ufc.br:2198 |
| Date | 04 December 2008 |
| Creators | Denise Lins de Sousa |
| Contributors | Josà Jeovà Siebra Moreira Neto, Cristiane Sà Roriz Fonteles, Grace Sampaio Teles da Rocha |
| Publisher | Universidade Federal do CearÃ, Programa de PÃs-GraduaÃÃo em Odontologia, UFC, BR |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFC, instname:Universidade Federal do Ceará, instacron:UFC |
| Rights | info:eu-repo/semantics/openAccess |
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