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Structural Analyses of a Human Valine Transfer RNA Gene and of a Transfer RNA Pseudogene Cluster

Two different cloned human DNA segments encompassing transfer RNA gene and pseudogene clusters have been isolated from a human gene library harbored in bacteriophage lambda Charon 4-A. One clone (designated as λhVal7) encompassing a 20.5-kilobase (Kb) human DNA insert was found to contain a valine transfer RNA_AAC gene and several Alu-like elements by Southern blot hybridization analysis and DNA sequencing with the dideoxyribonucleotide chain-termination method in the bacteriophage M13mp19 vector. Another lambda clone (designated as λhLeu8) encompassing a 14.3-Kb segment of human DNA was found to contain a methionine elongator transfer RNA_CAT pseudogene and other as yet unidentified transfer RNA pseudogenes.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc500850
Date12 1900
CreatorsLee, Mike Ming-Jen
ContributorsPirtle, Robert M., Norton, S. J., Wu, Edward Ming-chi, 1938-
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatviii, 100 leaves: ill., Text
RightsPublic, Lee, Mike Ming-Jen, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved.

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