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Transientní transfekce bezsérové buněčné kultury pomocí polyethyleniminů / Transient transfection of a serum free cell culture using polyethyleneimines

Master’s thesis deals with the transient transfection of the serum free animal cell culture using polyethyleneimines. In the theoretical part formation of recombinant DNA molecules, used expresion vectores, used DNA transfer and detection of recombinant proteins are discussed. The experimental part deals with efficiency of the polyethylenimine mediated transient transfection under various experimental conditions. 293HEK/EBNA cell line was chosen as an experimental model. First the most effective plasmide - pCEP4/SEAP was selected. Then three transfection methodes were tested: Muller (2005), Durocher et al. (2007) and Backliwal et al. (2008). The highest recombinant protein expresion was reached using the method of Backliwal et al. (2008).

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:216623
Date January 2010
CreatorsČutová, Michaela
ContributorsŠpanová, Alena, Ševčík, Mojmír
PublisherVysoké učení technické v Brně. Fakulta chemická
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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