Apolipoprotein B (apoB) is a key structural and functional protein of lipoproteins
and is synthesized constitutively in the liver. This study investigated the role of
microRNAs (miRNAs) in translational control of apolipoprotein B (apoB) mRNA and
protein synthesis. Using bioinformatic analysis, I identified two specific miRNAs
namely, miR-544 and miR-1202 with potential to interact with 3’ and 5’ UTR of apoB,
respectively. Using HepG2 cells as the model system, the effects of transfection of
exogenous miRNAs and inhibition of endogenous miRNAs were assessed on the
expression of apoB mRNA and protein synthesis, as well as apoB mRNA traffic into
cytoplasmic P-bodies. miR-544 induced a significant reduction in apoB mRNA
expression and protein synthesis while increasing the co-localization of apoB mRNA into
P-bodies. In contrast, transfection of miR-1202 increased apoB mRNA expression and
protein synthesis. In summary, these data demonstrate that specific miRNAs are involved
in translational control of apoB mRNA.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/42671 |
| Date | 20 November 2013 |
| Creators | Ansari Basir, Sahar |
| Contributors | Adeli, Khosrow |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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