Return to search

Localisation of equilibrative nucleoside transporter 3 (ENT3) in mouse brain

Adenosine is an essential purine nucleoside of particular importance within heart and brain. The widespread and diverse actions of adenosine, driven by activation of cell surface receptors, include regulation of sleep/arousal and neuroprotective properties. The mechanisms involved in regulating adenosine concentrations remain poorly understood but are critical to signaling pathways as they determine the availability of adenosine at corresponding receptors within the extracellular space. The equilibrative nucleoside transporter (ENT) family, bi-directional, Na+-independent nucleoside transporters, are key components in both the release and uptake of adenosine. This study has been conducted to investigate ENT3, a novel member of the ENT family. Our work has demonstrated ENT3 to be expressed throughout brain, located in cortex, cerebellum, striatum and hippocampus, at similar levels. Neurons and astrocytes, but not microglia, showed intracellular ENT3 localisation. This was confirmed by differential centrifugation, of cortex and cerebellum, which suggests ENT3 to be found within the cytoplasm.

Identiferoai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/30193
Date12 January 2015
CreatorsRoberts, Lauren Emilienne
ContributorsParkinson, Fiona E. (Pharmacology and Therapeutics), Miller, Donald (Pharmacology and Therapeutics) Triggs-Raine, Barbara (Biochemistry and Medical Genetics)
Source SetsUniversity of Manitoba Canada
Detected LanguageEnglish

Page generated in 0.0019 seconds