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Previous issue date: 2014-02-26 / Chronic lymphoproliferative disorders (DLPC) are lymphoid system diseases
characterized by the abnormal proliferation of mature lymphocytes that affect B
cells, T lymphocytes and NK cells. The aim of the study was to demonstrate the
relevance of immunophenotyping by flow cytometry in patients with prolonged
lymphocytosis and / or cytomorphological changes compatible with
lymphoproliferative diseases. In this study 460 patients (244 men and 216 women)
with DLPC were evaluated. Were analyzed by flow cytometry with a panel of
monoclonal antibodies consisting of CD3, CD4, CD5, CD8, CD10, CD19, CD22,
CD23, CD25, CD38, CD45, CD16/CD56, and HLADR heavy and light chains of
immunoglobulins. It also examines information regarding age, gender of patients
and laboratory data as leucocytes, cytomorphological analysis, platelet count and
hemoglobin determination. The results showed 398 cases of chronic
lymphoproliferative disorders and 62 of DLPC B cell lymphoproliferative diseases T.
B showed the following distribution : 253 cases of chronic lymphocytic leukemia
(CLL), 42 cases of multiple myeloma ( MM ), 37 cases of lymphoma non - Hodgkin
lymphoma in leukemic phase (NHL) , 17 cases of pro- B lymphocytic leukemia ( B -PLL), 15 cases of mantle cell lymphoma (MCL ), 12 cases of plasma cell leukemia (
PCL), 9 cases of lymphoma Burkitt (Linf B), 8 cases of leukemia villous cells ( LCV),
3 cases of splenic lymphoma with villous cells (LECV), a case of follicular lymphoma
(LF) and a Waldenstr?n macroglobulinemia ( MW). The diseases source NK / T
were 23 cases of peripheral T cell lymphoma (LCTP), 14 cases of T prolymphocytic leukemia (T -PLL), 10 cases of leukemia T of large granular
lymphocytes (LGL -T) 9 cases of leukemia cells of adult T (LCTA), 5 cases of
Sezary syndrome (SS) and a case of large granular NK leukemia (LGL -NK)
lymphocytes. In conclusion, the combined use of the monoclonal antibody panel
careful cytomorphological analysis was shown to be essential in immune diagnosis
and classification of chronic lymphoproliferative disorders. This study was approved
by the IRB - HUOL under number 356 / 09 / Os transtornos linfoproliferativos cr?nicos (DLPC) s?o doen?as do sistema linfoide
caracterizadas pela prolifera??o anormal de linf?citos maduros que acometem
c?lulas B, linf?citos T e c?lulas NK. O objetivo do estudo foi demonstrar a
relev?ncia da imunofenotipagem por citometria de fluxo em pacientes portadores de
linfocitose prolongada e/ou altera??es citomorfol?gicas compat?veis com doen?as
linfoproliferativas. Neste estudo foram avaliados 460 pacientes (244 homens e 216
mulheres) com DLPC. Foram analisados por citometria de fluxo com um painel de
anticorpos monoclonais constitu?do por CD3, CD4, CD5, CD8, CD10, CD19, CD22,
CD23, CD25, CD38, CD45, CD16/CD56, HLADR e cadeias leves e pesadas de
imunoglobulinas. Foram tamb?m investigadas informa??es referentes ? idade, ao
g?nero dos pacientes e aos dados laboratoriais como: leucometria, an?lise
citomorfol?gica, contagem de plaquetas e determina??o da hemoglobina. Os
resultados demonstraram 398 casos de doen?as linfoproliferativas cr?nicas B e 62
de DLPC de c?lula T. As doen?as linfoproliferativas B apresentaram a seguinte
distribui??o: 253 casos de leucemia linfoc?tica cr?nica (LLC), 42 casos de mieloma
m?ltiplo (MM), 37 casos de linfoma n?o Hodgkin em fase leuc?mica (LNH), 17
casos de leucemia pr?-linfoc?tica B (LPL-B), 15 casos de linfoma de c?lulas do
manto (LCM), 12 casos de leucemia de c?lulas plasm?ticas (LCP), 9 casos de
linfoma de Burkitt (Linf B), 8 casos de leucemia de c?lulas vilosas (LCV), 3 casos de
linfoma espl?nico de c?lulas vilosas (LECV), um caso de linfoma folicular (LF) e um
de macroglobulinemia de Waldenstr?n (MW). As doen?as de origem T/NK foram:
23 casos de linfoma de c?lulas T perif?ricas (LCTP), 14 casos de leucemia pr?linfoc?tica T (LPL-T), 10 casos de leucemia de grandes linf?citos T granulares (LGLT), 9 casos de leucemia de c?lulas T do adulto (LCTA), 5 casos de S?ndrome de
Sezary (SS) e um caso de leucemia de grandes linf?citos NK granulares (LGL-NK).
Em conclus?o, o uso do painel de anticorpos monoclonais combinado ? an?lise
citomorfol?gica cuidadosa mostrou-se essencial no diagn?stico e classifica??o
imune das doen?as linfoproliferativas cr?nicas. O presente estudo foi aprovado pelo
CEP HUOL sob n?mero 356/09
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13415 |
| Date | 26 February 2014 |
| Creators | Paiva, Aldair de Sousa |
| Contributors | CPF:10861203453, http://lattes.cnpq.br/0091662650633339, R?go, Am?lia Cinthia Meneses do, CPF:78914450368, http://lattes.cnpq.br/3240686272929972, Cavalcanti J?nior, Geraldo Barroso |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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