A dietary level of Aroclor 1254 (100 ppm) was fed
to rainbow trout (Salmo gairdneri) for 15 weeks to
determine the effects on hepatic microsomal enzyme induction.
Fish were also fed combined polychlorinated
biphenyl (PCB) (100 ppm) and cyclopropene fatty acids
(CPFA) (50 ppm) to determine the effects on mixed
function oxidase (MFO) induction.
Dietary PCBs markedly induced the microsomal activities
of 7-ethoxyresorufin O-deethylase, 7-ethoxy-coumarin
O-deethylase, and benzo(a)-pyrene monooxygenase.
Ethoxyresorufin O-deethylase activity continued
to increase to a level 77-fold higher than control at
week 15. Ethoxycoumarin O-deethylase and benzo(a)-pyrene monooxygenase activities increased to 7.1-fold
and 48-fold over control at week 9 and then slightly
decreased to 6.8-fold and 45-fold over control at week
15, respectively. Cytochrome P450 values remained
approximately 2-fold above controls from week 5 through
week 15. At weeks 1 and 3, cytochrome P450 levels were
not significantly different from control.
Ethoxyresorufin O-deethylase, ethoxycoumarin
O-deethylase, and benzo(a)pyrene monooxygenase activities
in the combined PCB and CPFA-fed trout were significantly
higher than in controls and CPFA-fed fish,
and significantly lower than in PCB-fed fish. There
was no significant difference in cytochrome P450 levels
after week 5.
This is the first time dietary PCBs have been
shown to induce the MFC system in PCB-fed rainbow
trout. / Graduation date: 1980
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27095 |
| Date | 05 May 1980 |
| Creators | Voss, Sherri Denise |
| Contributors | Hendricks, Jerry D. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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