Prostaglandin E2 (PGE2) is an immunosuppressive factor secreted by the murine Lewis lung carcinoma (LLC). This factor can help to insure the survival of a tumor by suppressing the functions of lymphocytes and macrophages in their defense against tumors.Four weekly assays of macrophage tumoristatic activity from tumor-bearing mice were performed during the course of tumor development. Macrophages from the peritoneal cavity were cultured with LLC cells and after 48 hours the LLC growth was measured.The results of this study showed that macrophages from tumorbearing mice were suppressed in their ability to halt LLC growth in vitro. This suppression could be prevented by the use of indomethacin, a prostaglandin synthesis inhibitor. Indomethacin administration to tumor-free mice also enhanced their macrophage tumoristatic activities suggesting that the tumor was not the exclusive source of prostaglandin which suppressed anti-tumor immunity. Finally, indomethacin-fed tumor-bearing mice had smaller tumors than did mice not fed indomethacin.Ball State UniversityMuncie, IN 47306
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/182894 |
| Date | 03 June 2011 |
| Creators | Estep, Clayton E. |
| Contributors | Young, M. Rita |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | 27 leaves ; 28 cm. |
| Source | Virtual Press |
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