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Kapilární elektroforéza s duální optickou a bezkontaktní vodivostní detekcí. / Capillary electrophoresis with dual optical and contactless conductometric detection.

This work deals with dual detection of organic and inorganic analytes after separation by capillary zone electrophoresis. In the first part, two types of hydrodynamic sampling are tested. Standard hydrodynamic sampling most often used in laboratory-made electrophoretic apparatus, based on lifting the vessel with the sample, in which the sampling end of the capillary is immersed, and a new method based on increasing the pressure in the sampling vessel without moving the capillary. This sampling procedure minimizes experimenter activity because it is controlled by software. Experimenter only changes vessel containing the sample solution for one with separation electrolyte. The experimental parameters, the sampling time and pressure, are optimized to achieve maximum separation efficiency and adequate detection sensitivity. In the second part of the work, the developed method is tested for the separation of amino-acids in a biological sample (urine).

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:324122
Date January 2013
CreatorsKadlecová, Tereza
ContributorsOpekar, František, Jelínek, Ivan
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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