Understanding how animals detect and discriminate different qualities of light is a key component of the study of visual ecology. My research investigated the use of three electrophysiological methods to assess the neuronal mechanisms involved in spectral and polarization sensitivity in one species of salmonid, the rainbow trout (Onchorynchus mykiss). 1 examined the neuronal mechanisms underlying polarization sensitivity using electroretinograms (ERG) and optic nerve compound action potential (CAP) recordings. Chromatic adaptation and pharmacological techniques were used to reveal opponent interaction at the cone-horizontal cell level and to provide the first evidence of retinal processing of polarization sensitivity. To facilitate additional research more suited to the exploration of neural networks and signaling, I developed the protocols and techniques necessary to investigate the spectral sensitivity of rainbow trout using whole-cell patch clamp (WPC) electrophysiology, and produced the first assessment of the ultraviolet component of spectral sensitivity in a vertebrate using this technique.
| Identifer | oai:union.ndltd.org:uvic.ca/oai:dspace.library.uvic.ca:1828/2223 |
| Date | 19 February 2010 |
| Creators | Anderson, Leslie Gayle |
| Contributors | Hawryshyn, Craig W. |
| Source Sets | University of Victoria |
| Language | English, English |
| Detected Language | English |
| Type | Thesis |
| Rights | Available to the World Wide Web |
Page generated in 0.0013 seconds