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Impact of macronutrient restriction and probiotic supplementation on protein synthesis and growth in a piglet model of dextran sulphate-induced colitis

A dextran sulphate (DS) model of ulcerative colitis was used to examine the effects of macronutrient restriction with (MR+PRO) and without (MR) probiotic supplementation (VSL#3RTM) on protein metabolism and growth. MR and MR+PRO decreased weight and chest circumference gain, but had no effect on linear growth of piglets. MR decreased the protein fractional synthesis rate (FSR) of liver, masseter, longissimus dorsi, colon, as well as plasma albumin, measured by stable isotope tracer L-[ring-2H 5]phenylalanine. MR+PRO increased the FSR of hepatic proteins by greater than 70% and increased both the FSR and absolute synthesis rate (ASR) of the total plasma protein pool, albumin and fibrinogen. Over 11 days, MR+PRO piglets showed a smaller decrease in plasma total protein concentrations than WN piglets, and maintained higher albumin levels than either WN or MR piglets. These findings highlight the importance of adequate nutrition during gastrointestinal inflammation and show that when food intake is decreased, probiotics stimulate liver protein synthesis and increase the acute phase protein response, similar to the well-nourished state.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.99178
Date January 2006
CreatorsFraser, Keely Gabrielle.
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (School of Dietetics and Human Nutrition.)
Rights© Keely Gabrielle Fraser, 2006
Relationalephsysno: 002573624, proquestno: AAIMR28487, Theses scanned by UMI/ProQuest.

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