Concentration of microorganisms from a sample volume would increase the limits of detection of samples used for rapid-detection methods. Rapid detection methods are is advantageous for the food industry to rapidly test for bacteria in order release products on a timely basis. Ultrasonic concentration was considered a promising method for manipulation of microorganisms. An ultrasonic chamber consisting of parallel piezoceramic discs with a reticulated polyurethane foam mesh was used to concentrate Saccharomyces cerevisiae yeast and Escherichia coli bacteria. The concentration of yeast was seen to increase by 200% (from 8.0 x 104 cells mL-1 to 2.4 x 105 cells mL-1) while almost zero concentration of bacteria was observed. The poor concentration effect seen with the smaller microorganisms was explained by the volume dependent acoustic radiation force exerted on the particles; the concentration forces are 1,000 times smaller for a 1 μm bacteria cell versus a 10 μm yeast cell.
| Identifer | oai:union.ndltd.org:uky.edu/oai:uknowledge.uky.edu:bae_etds-1006 |
| Date | 01 January 2012 |
| Creators | Mullins, Samuel J |
| Publisher | UKnowledge |
| Source Sets | University of Kentucky |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations--Biosystems and Agricultural Engineering |
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