Resumo: Não disponível / Abstract: Uncaria tomentosa (Willd.) DC (Rubiaceae) is a large woody vine that is native to the Amazon and Central American rainforests and is widely used in traditional medicine for its immunomodulatory and anti-inflammatory activities. The present work used in vivo immunotoxic and in vitro immunomodulatory experiments to investigate the effects of a pentacyclic oxindole alkaloid extract from U. tomentosa bark on lymphocyte phenotype, Th1/Th2 cytokine production, cellular proliferation and cytotoxicity. For the in vivo immunotoxicity testing, BALB/c male mice were treated once a day with 125, 500 or 1250 mg/kg of U. tomentosa extract for 28 days. For the in vitro protocol, lymphocytes were cultured with 10 to 500 μL/mL of the extract for 48 h. The extract increased the cellularity of splenic white pulp and the thymic medulla and increased the number of T helper lymphocytes and B lymphocytes. Also, a large stimulatory effect on lymphocyte viability was observed. However, mitogen-induced T lymphocyte proliferation was significantly inhibited at higher concentrations of U. tomentosa extract. Furthermore, an immunological polarization toward a Th2 cytokine profile was observed. These results suggest that the U. tomentosa aqueous-ethanol extract was not immunotoxic to mice and was able to modulate distinct patterns of the immune system in a dose-dependent manner / Orientador: Rosa Marlene Viero / Coorientador: Alexandrina Sartori / Banca: Carla Adriene da Silva Franchi / Banca: Ana Lúcia Tozzi-Spinardi Barbisan / Banca: Isis Machado Hueza / Banca: Efigênia Queiroz de Santana / Doutor
| Identifer | oai:union.ndltd.org:UNESP/oai:www.athena.biblioteca.unesp.br:UEP01-000638839 |
| Date | January 2011 |
| Creators | Domingues, Alexandre. |
| Contributors | Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina. |
| Publisher | Botucatu : [s.n.], |
| Source Sets | Universidade Estadual Paulista |
| Language | Portuguese |
| Detected Language | English |
| Type | text |
| Format | 159 f. |
| Relation | Sistema requerido: Adobe Acrobat Reader |
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