A reverse-phase high pressure liquid chromatographic method for the quantitative analysis of unchanged ascorbic acid in human urine is described. Selection of an appropriate mobile phase and discussion of some of the analysis problems are presented. Twenty-four hour ascorbate excretion profiles from two subjects were determined. Standard redox titration procedures were used to corroborate the chromatographic method. When compared to classical titration or colorimetric redox procedures, the new assay features straightforward sample preparation and improved sensitivity.Ball State UniversityMuncie, IN 47306
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/181267 |
| Date | 03 June 2011 |
| Creators | Coffin, Robert D. |
| Contributors | Wagner, Eugene S. |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | iv, 48 leaves : ill. ; 28 cm. |
| Source | Virtual Press |
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