Recently, viral hemorrhagic septicemia virus (VHSV IVb) was associated with several walleye (Sander vitreus) mortality events in the Great Lakes. To examine the effects of route, strain-variation and temperature, walleye were experimentally infected with VHSV IVb using intraperitoneal (i.p.)-injection (102-108 pfu/fish) and immersion (w.; 1.4 x 107 virions mL-1). Walleye were relatively resistant to experimental infection with VHSV IVb, regardless of route or water temperature. High cumulative mortality (64-100%) and severe gross lesions associated with VHSV-IVb infection were only evident in fish i.p.-injected with 108 pfu at 12°C, which had mild to moderate, multifocal necrosis of several tissues including the gill and heart. There were significant differences in mortality between four walleye strains following i.p.-infection. Viral antigen was found in both i.p. and w.-exposed walleye using immunohistochemistry, mostly within the gill and skin epithelium of w.-exposed fish. VHSV IVb was detected in walleye tissues from 6-21 d post-infection using RT-qPCR. / Great Lakes Fisheries Commission and NSERC
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OGU.10214/3563 |
| Date | 04 May 2012 |
| Creators | Grice, Jessica |
| Contributors | Lumsden, John |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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