Magistrinio darbo metu sukonstruotos mielių plazmidės, turinčios pelių poliomos viruso pagrindinį kapsidės baltymą VP1 su jame įterptais hepatito C viruso apvalkalo baltymų peptidais. Šiomis plazmidėmis buvo transformuotos S. cerevisiae mielės. Transformuotos mielės sintetina chimerinius baltymus: MPyV-VP1-BC-E2 [384-414], MPyV-VP1-HI-E2 [384-414], MPyV-VP1-BC-E1 [310-329], MPyV-VP1-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447], MPyV-VP1-HI-E2 [412-i447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-i447], MPyV-VP1-BC-E2 [412-447]-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447]-HI-E1 [310-329]. Į virusus panašias daleles (VPD) renkasi tik MPyV-VP1-BC-E2 [384-414], MPyV-VP1-HI-E2 [384-414], MPyV-VP1-BC-E1 [310-329] baltymai. Kiti baltymai: MPyV-VP1-BC-E1 [310-329], MPyV-VP1-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447], MPyV-VP1-HI-E2 [412-i447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-i447], MPyV-VP1-BC-E2 [412-447]-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447]-HI-E1 [310-329] nesirinko į VPD. / In the present study, plasmids for expression of major capsid proteins VP1 of murine polyomavirus with inserted sequences from Hepatitis C virus envelope proteins in yeast S. cerevisiae were constructed. The plasmids were used to transform yeast cells. The transformed yeast produced proteins: MPyV-VP1-BC-E2 [384-414], MPyV-VP1-HI-E2 [384-414], MPyV-VP1-BC-E1 [310-329], MPyV-VP1-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447], MPyV-VP1-HI-E2 [412-i447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-i447], MPyV-VP1-BC-E2 [412-447]-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447]-HI-E1 [310-329]. Virus-like particles (VLPs) composed MPyV-VP1-BC-E2 [384-414], MPyV-VP1-HI-E2 [384-414], MPyV-VP1-BC-E1 [310-329]. Other proteins: MPyV-VP1-BC-E1 [310-329], MPyV-VP1-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447], MPyV-VP1-HI-E2 [412-i447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-447], MPyV-VP1-BC-E1 [310-329]-HI-E2 [412-i447], MPyV-VP1-BC-E2 [412-447]-HI-E1 [310-329], MPyV-VP1-BC-E2 [412-i447]-HI-E1 [310-329] did not form VLPs.
Identifer | oai:union.ndltd.org:LABT_ETD/oai:elaba.lt:LT-eLABa-0001:E.02~2007~D_20101125_183215-91788 |
Date | 25 November 2010 |
Creators | Sabaliauskaitė, Rasa |
Contributors | Sasnauskas, Kęstutis, Vilnius University |
Publisher | Lithuanian Academic Libraries Network (LABT), Vilnius University |
Source Sets | Lithuanian ETD submission system |
Language | Lithuanian |
Detected Language | Unknown |
Type | Master thesis |
Format | application/pdf |
Source | http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20101125_183215-91788 |
Rights | Unrestricted |
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