The aim of this thesis was to analyze populations of pigs of breed Czech Large White and to evaluate their genetic variability and diversity. Eleven single-nucleotide polymorphisms (SNPs) were analyzed in porcine candidate genes. In total, 139 individuals from two populations of different status in breeding program were evaluated. Polymorphisms were genotyped by PCR-RFLP in loci: HMGCR, TCF7L2, MC4R, EDG4, HFABP, FTO, LEPR6, LEPR18, PDK4, PLINHin, PLINNIa. Population-genetic analysis was done in program POPGENE 1.31. All observed loci were polymorphic. The occurrence of rare allele T of locus EDG4 in population of breeding program was considered. Genetic diversity in population of commercial breed was higher than diversity of breeding population. All observed populations were in Hardy-Weinberg equation.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:179673 |
| Date | January 2014 |
| Creators | Černošková, Barbora |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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