The purpose is to isolate a natural compound, which shows a high activity against Candida albicans, from plant, Vincetoxicum stocksii. Bio-Assay, Thin layer Chromatography, Column Chromatography, TLC bio Assay, and other extraction techniques are used in order to isolate the active compound. First, bio assay technique is carried out on the crude gum. Next, several flash chromatography columns are carried out in order to isolate the target compound, which has a Rf value of ~0.53 in 10:1 DCM/methanol solvent mixture. The TLC bioassay technique is also carried out in order to confirm the hypothesis that the target compound is indeed active.
Identifer | oai:union.ndltd.org:GEORGIA/oai:digitalarchive.gsu.edu:chemistry_theses-1008 |
Date | 30 April 2008 |
Creators | Momin, Vasim Rasul |
Publisher | Digital Archive @ GSU |
Source Sets | Georgia State University |
Detected Language | English |
Type | text |
Format | application/pdf |
Source | Chemistry Theses |
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