Industrial hemp (Cannabis sativa) is a highly productive crop that is well suited to
cultivation in Canada. To better understand the development of bast (phloem)
fiber secondary walls and to facilitate reverse genetics screening for improved
germplasm, I undertook two sets of microarray experiments. The first compared
transcript expression in stem peels at three positions along the length of the stem.
The second set of microarray experiments compared transcript expression in
adjacent tissue layers along the radial axis of the stem. The transcripts that were
enriched in fiber-producing tissues in both studies were consistent with a dynamic
program of cell wall deposition. Detailed qRT-PCR analysis of specific
lignification genes identified the best targets for reverse genetics. Finally, as a
first step towards establishing a virally induced gene silencing (VIGS) system, I
identified viruses that produced visual symptoms of infection, although qRT-PCR
failed to confirm the infection / Plant Biology
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/812 |
| Date | 06 1900 |
| Creators | Koziel, Susan P. |
| Contributors | Deyholos, Michael (Biological Sciences), Gallin, Warren J. (Biological Sciences), Scarpella, Enrico (Biological Sciences), Strelkov, Stephen (Faculty of Agricultural, Food and Nutritional Science) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 4634377 bytes, application/pdf |
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