This work concentrated on isolation of novel hop transcription factors from bHLH, bZIP, MYB, and WRKY families involved in the regulation of lupulin flavonoid pathways, followed by their structural and functional analysis. Structural analyses included bioinformatic approaches to elucidate gene organization, domain structure of the putative protein products, and potential post-translational modifications. I performed site-directed mutagenesis to disclose the role of phosphorylation sites in HlbZIP1A stability. Further, this work determined protein-DNA interactions for obtained TFs, giving support to the binding of MYB-bHLH-WDR complexes to the promoter of chalcone synthase H1, a key enzyme of the lupulin flavonoid pathways. Employing bioinformatic approaches, quantitative RT-PCR and transient co-expression, I pointed out chalcone synthase H1 as a regulatory crossroads in the metabolic (flavonoid) responses during hop stunt viroid pathogenesis.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:151840 |
| Date | January 2013 |
| Creators | FÜSSY, Zoltán |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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