Riboflavin status was determined in 16 Oriental
males and females who were residing off the Oregon State
University campus. Before and after these subjects had
received 10 mg of riboflavin daily for 7 days, we measured
erythrocyte glutathione reductase (EGR) activity with and
without FAD added in vitro and erythrocyte total
riboflavin levels (RBC B-2). Riboflavin status was
assessed by the EGR activity coefficient (EGRAC) (EGR
activity with FAD added in vitro / EGR activity without
FAD added in vitro). The subjects' dietary intake of
riboflavin, protein and calories was estimated from their
self chosen diets which were recorded for 3 days before
the riboflavin supplementation. Both sex groups had
adequate mean levels of riboflavin, protein and calories
in their diets. Further, none of the subjects had an
intake less than two-thirds of the recommended dietary
allowances (FNB, 1980) for riboflavin. Before riboflavin supplementation 5 of the 9 males and 2 of the 7 females
had EGRAC greater than or equal to 1.2, suggesting that
they were at high risk of marginal riboflavin deficiency,
and to have a normal EGRAC they may need an intake of
riboflavin greater than the present recommended allowance.
In all of the subjects, EGRAC decreased in response
to the riboflavin supplements. Additionally, the subjects'
RBC B-2, EGR basal activity (without FAD added in vitro)
and FAD stimulated EGR activity increased significantly (P < 0.01) after 7 days of riboflavin supplementation.
Although the RBC B-2 concentrations were lower than those
reported by Bessey, Horwitt and Love (1956) and Bamji
(1969), this measurement correlated significantly with
EGRAC (r = -0.64, P < 0.01), EGR basal activity (r = 0.74,
P < 0.01) and FAD stimulated EGR activity (r = 0.57, P < 0.01). Dietary riboflavin intake whether expressed as
total riboflavin intake per day or riboflavin intake per
1000 Kcal was not correlated to any of these biochemical
tests made before riboflavin supplementation. RBC B-2
values obtained from these 16 Orientals were similar to
those obtained in 5 Caucasian subjects, indicating that
these low values were not due to genetic differences. / Graduation date: 1986
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/27131 |
| Date | 17 October 1985 |
| Creators | Yeh, Shih-ya |
| Contributors | Miller, Lorraine T. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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