Chi-man Au. / Thesis (M.Ph.)--Chinese University of Hong Kong. / Bibliography: leaves 74-90. / ACKNOWLEDGEMENTS --- p.ii / Chapter I --- INTRODUCTION --- p.1 / Chapter II --- LITERATURE REVIEW --- p.4 / Chapter III --- MATERIALS AND METHODS / Chapter A --- Study of the growth pattern of the fruit body of Volvariella volvacea / Chapter 1 --- Measurement of growth rate of fruit bodies --- p.24 / Chapter 2 --- Study of the dependence on the cap and mycelium for development of fruit bodies --- p.26 / Chapter 3 --- Study of stipe elongation --- p.26 / Chapter B --- A preliminary study of the cell wall of Volvariella volvacea / Chapter 1 --- Preparation of the cell wall --- p.27 / Chapter 2 --- Calculation of the percentage weight of cell wall --- p.28 / Chapter 3 --- Chemical fractionation of the cell wall --- p.29 / Chapter 4 --- Determination of glucose content of the cell wall --- p.29 / Chapter 5 --- Determination of N-acetylglucosaimine content --- p.30 / Chapter C --- A study of the properties of chitin synthetase in Volvariella volvacea / Chapter 1 --- Preparation of enzyme fraction --- p.31 / Chapter 2 --- Assay of enzyme activity --- p.32 / Chapter 3 --- Protein determination --- p.33 / Chapter 4 --- Determination of optimal temperature of incubation --- p.34 / Chapter 5 --- Determination of the optimal pH --- p.34 / Chapter 6 --- Determination of the variation of product incorporation with time --- p.35 / Chapter 7 --- Determination of the stability of the enzyme --- p.35 / Chapter 8 --- Determination of the Michaelis-Menten constants of the enzyme in the absence and presence of N-acetylglucosamine --- p.35 / Chapter D --- To find the change in specific activity of chitin synthetase from different stages of the fruit bodies --- p.36 / Chapter IV --- RESULTS / Chapter A --- Study of the growth pattern of the fruit bodies of Volvariella volvacea / Chapter 1 --- Measurement of the growth rate of the fruit bodies --- p.37 / Chapter 2 --- The dependance on the cap and mycelium for development of the fruit bodies --- p.37 / Chapter 3 --- Study of stipe elongation --- p.40 / Chapter B --- Study of the cell wall of Volvariella volvacea / Chapter 1 --- Percentage weight of the cell wall --- p.40 / Chapter 2 --- Chemical fractionation of the cell wall --- p.42 / Chapter 3 --- The glucose content of the cell wall --- p.42 / Chapter 4 --- The N-acetylglucosamine content of the cell wall --- p.46 / Chapter C --- The properties of chitin synthetase from the mycelium of Volvariella volvacea / Chapter 1 --- The optimal temperature of incubation --- p.46 / Chapter 2 --- The optimal pH of incubation --- p.49 / Chapter 3 --- The variation of product incubation with time --- p.49 / Chapter 4 --- The stability of chitin synthetase in Volvariella volvacea --- p.49 / Chapter 5 --- The Michaelis-Menten constants (Km) of chitin synthetase in the absence and pre-sence of N-acetylglucosamine --- p.53 / Chapter D --- The change in specific activity of chitin synthetase from fruit bodies of different developmental stages --- p.53 / Chapter V --- DISCUSSION --- p.62 / Chapter VI --- SUMMARY --- p.72 / Chapter VII --- REFERENCES --- p.74
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321160 |
| Date | January 1979 |
| Contributors | Au, Chi-man., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | S.n.] |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | print, vi, 90 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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