The development of a technique for producing a pure Bacteroides fragilis infection in mice is described. The infection produces large subcutaneous abscesses at the site of bacterial injection in approximately 90% of the mice. The abscess can be seen as an obvious swelling within 5-7 days post-injection. The infection was initiated by injection of pure cultures grown in a semisolid medium. Similar infections were also produced with pure cultures of B. distasonis, B. ovatus, B. thetaiotaomicron, and B. vulgatus. / Ph. D.
| Identifer | oai:union.ndltd.org:VTETD/oai:vtechworks.lib.vt.edu:10919/39010 |
| Date | 01 August 2012 |
| Creators | Walker, Clay B. |
| Contributors | Microbiology, Wilkins, Tracy D., Moore, Walter E. C., Cummins, C. S., Gregory, Eugene M., Krieg, Noel R. |
| Publisher | Virginia Tech |
| Source Sets | Virginia Tech Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Dissertation, Text |
| Format | v, 133 leaves, BTD, application/pdf, application/pdf |
| Rights | In Copyright, http://rightsstatements.org/vocab/InC/1.0/ |
| Relation | OCLC# 40274359, LD5655.V856_1977.W355.pdf |
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