Key Words: Thrombosis, Vascular Graft, Microfluidics, Wall Shear Stress / Vascular grafts are essential for the management of cardiovascular disease. However, the lifesaving potential of these devices is undermined by thrombosis arising from material and flow interactions on the blood contacting surface. To combat this issue, the use of antithrombogenic coatings has emerged as a promising strategy for modulating blood and graft interaction in vivo. Although an important determinant of graft performance, hemodynamics are frequently overlooked in the in vitro testing of coatings and their translatability remains poorly understood. We address this limitation with a microscale platform that incorporates vascular prosthesis and coatings with tuneable flow and surface conditions in vitro. As a proof of concept, we use the platform to test the thrombogenic performance of a novel class of lubricant infused (LIS) and antibody lubricant infused (anti-CD34 LIS) coated ePTFE vascular grafts in the presence of arterial wall shear stress, with and without the presence of endothelial cells. Our findings suggest lubricant infused coated ePTFE vascular grafts are thromboresistant under flow and may have potential for in vivo arterial grafting applications. It is moreover apparent that the microscale properties of the device could be advantageous for the testing and translation of novel antithrombogenic coatings or blood contacting prosthesis in general. / Thesis / Master of Applied Science (MASc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/27511 |
| Date | January 2022 |
| Creators | Bot, Veronica |
| Contributors | Didar, Tohid, Biomedical Engineering |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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