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Intracellular Location of Carotenoid Pigments in Yeast-Phase Cells of Wangiella Dermatitidis and Cell Wall Morphology After Enzyme Treatment

Carotenoid pigments in W. dermatitidis, the first pathogenic, dematiaceous fungus in which carotenoid pigments nave been reported, are located primarily (81%) in lipid organelles which floated on the surface of the supernatant fraction of lysed cells. Pigment in this fraction could be extracted with ethyl ether without prior treatment with acetone indicating the pigment is unbound in the lipid organelle. Eight percent remains after exhaustive ether extraction and is recovered after the sample is treated with acetone indicating this fraction is non-covalently bound to proteins in the membranes associated with the lipid organelle. The remaining pigment (about 12%) represents contamination of the supernatant with the lipid organelles.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798104
Date12 1900
CreatorsFoster, Linda Ann
PublisherUniversity of North Texas
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvii, 127 leaves : ill., Text
RightsPublic, Foster, Linda Ann, Copyright, Copyright is held by the author, unless otherwise noted. All rights

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