The goal of this thesis was to examine the sources and ecological behaviour of genotoxic organics in the St. Lawrence river system. A rapid and effective version of the SOS Chromotest was developed to accomplish the task of genotoxicity assessment. The method, validated with standard reference materials, is particularly well suited to complex environmental extracts. The endpoint investigated throughout the thesis, SOS genotoxicity, is empirically related to more familiar endpoints such as mutagenicity and carcinogenicity. Analyses of literature data confirmed correlations between SOS genotoxicity and both Ames/Salmonella mutagenicity and animal carcinogenicity. Analyses of industrial and municipal wastewaters revealed that organic and inorganic chemical production facilities, metal refining and founding facilities and petroleum refineries release high potency wastes. Loading values indicate that low potency sources (e.g. municipal wastewater and pulp & paper facilities) often have exceptionally high loadings. A substantial portion (2.3-99.8%) of industrial genotoxins are associated with suspended particulates. Sorption partition efficients (K$ sb{ rm d-genotox}$) indicate that municipal wastewater and pulp & paper facilities release particularly soluble genotoxins. Extracts of suspended particulate matter collected near industrial discharges are direct-acting. Bottom sediment extracts are less potent and usually require S9 metabolic activation. Genotoxicity of both bottom sediment and suspended particulates are empirically related to the genotoxicity of regional industrial emissions. Direct-acting substances in the water column appear to be less stable and may be converted to more stable pro-genotoxic substances present in the bottom sediments. A mass balance of genotoxicity for St. Lawrence surface waters revealed that domestic, rather than industrial sources make the largest contribution to observed genotoxicity. The importance of domestic wastes was confirmed
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.40281 |
| Date | January 1995 |
| Creators | White, Paul Andrew |
| Contributors | Rasmussen, Joseph B. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001484910, proquestno: NN12510, Theses scanned by UMI/ProQuest. |
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