Viruses may invade animals, plants, or bacteria, and are considered obligate parasites because they lack a complete enzyme system of their own. Reproduction occurs when the virus entity makes use of a cell's synthetic machinery to direct the synthesis of specialized particles, the virions, and transfers them to other cells. The purpose of this study was to investigate and develop methods to isolate viruses chicken embryos and bacteriophage.Viruses were concentrated from water sources following procedures outlined by Berg (1971) using standard Millipore filtering units. Virus suspensions were eluted with a beef extract solution and then inoculated into the three host systems. Growth was determined by the presence of cytopathic effects on the agar overlay or in the chicken on the chorioallantoic membrane.Cytopathic effects occurred in tissue cultures following inoculation with water samples from two sites on two consecutive sampling dates. Virus recovery in chicken embryos was 45.5% when cytopathic effects and death rates were analyses were combined. When bacteria were employed, viruses were recovered in 42.8% of samples tested.Ball State UniversityMuncie, IN 47306
| Identifer | oai:union.ndltd.org:BSU/oai:cardinalscholar.bsu.edu:handle/181231 |
| Date | 03 June 2011 |
| Creators | Loucks, Kathryn |
| Contributors | Nash, Peter |
| Source Sets | Ball State University |
| Detected Language | English |
| Format | vi, 49 leaves : map ; 28 cm. |
| Source | Virtual Press |
| Coverage | n-us-in |
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