For this research, the abnormality of tissue cultured plantlets,vitrification, was examined in Gypsophila paniculata.Measurement of the relative water content and water saturation deficit of plantlets in culture revealed that vitrified plantlets contain relatively more water and less air spaces than non-vitrified plantlets.The effect of relative humidity on vitrification and growth was investigated using a variety of methods.From the results found, it was determined the defining characteristic of vitrified plantlets is water filled intercellular spaces. It was also determined that the primary cause of vitrification is high relative humidity resulting in a lack of transpiration in vitro but that other factors such as unbalanced mineral nutrition or high medium cytokinin can exacerbate vitrification.Further research in tissue culture may investigate the influence of relative humidity on plant growth and morphology, the mechanism by which plants exclude water from their intercellular spaces and refine in vitro tissue mineral analysis as a means by which critical mineral concentrations can be determined. / Doctor of Philosophy (PhD)
| Identifer | oai:union.ndltd.org:ADTP/235068 |
| Date | January 1999 |
| Creators | Gribble, Karleen D., University of Western Sydney, Hawkesbury, Faculty of Science and Technology |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
| Source | THESIS_FST_HPS_Gribble_K.xml |
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