Sun Baizhong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 75-79). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ABSTRACT (IN CHINESE) --- p.ii / TABLE OF CONTENTS --- p.iii / LIST OF FIGURES --- p.v / LIST OF TABLES --- p.vii / ABBREVIATIONS --- p.vi / ACKNOWLEDGEMENT --- p.ix / DECLARATION --- p.x / Chapter CHAPTER ONE --- INTRODUCTION / Chapter 1.1 --- Polysaccharides --- p.1 / Chapter 1.2 --- Pharmacological importance of polysaccharides --- p.2 / Chapter 1.3 --- Polysaccharides from Ganoderma Lucidum --- p.3 / Chapter 1.4 --- Characterization of polysaccharides --- p.3 / Chapter 1.5 --- Matrix-assisted laser desorption / ionization --- p.5 / Chapter 1.6 --- Matrix-assisted laser desorption / ionization of polysaccharides --- p.8 / Chapter 1.7 --- Outline of the project --- p.10 / Chapter CHAPTER TWO --- INSTRUMENTATION AND EXPERIMENTAL / Chapter 2.1 --- Time-of-flight mass spectrometry --- p.12 / Chapter 2.1.1 --- Instrumentation --- p.15 / Chapter 2.1.1.1 --- Laser system --- p.16 / Chapter 2.1.1.2 --- Ion source --- p.16 / Chapter 2.1.1.3 --- Reflector --- p.19 / Chapter 2.1.1.4 --- Detector --- p.19 / Chapter 2.1.1.5 --- Data acquisition and manipulation --- p.20 / Chapter 2.2 --- Ultraviolet-visible spectrometer --- p.21 / Chapter CHAPTER THREE --- OPTIMIZATION OF MALDI CONDITIONS FOR POLYSACCHARIDE ANALYSIS USING DMSO AS SOLVENT / Chapter 3.1 --- Introduction --- p.22 / Chapter 3.2 --- Experimental --- p.25 / Chapter 3.2.1 --- Selection of matrix materials --- p.26 / Chapter 3.2.2 --- Selection of co-matrix materials --- p.26 / Chapter 3.2.3 --- Ratios of matrix-to-analyte --- p.26 / Chapter 3.2.4 --- Effect of sample drying temperature --- p.27 / Chapter 3.2.5 --- Sample loading methods --- p.27 / Chapter 3.3 --- Results and discussion --- p.27 / Chapter 3.3.1 --- Selection of matrix materials --- p.28 / Chapter 3.3.2 --- Selection of co-matrix materials --- p.30 / Chapter 3.3.3 --- Effect of matrix-to-co-matrix ratio --- p.32 / Chapter 3.3.4 --- Effect of sample drying temperature --- p.37 / Chapter 3.3.5 --- Effect of matrix-to-analyte ratio --- p.39 / Chapter 3.3.6 --- Evaluation of sample preparation protocols --- p.43 / Chapter 3.4 --- Conclusion --- p.46 / Chapter CHAPTER FOUR --- MOLECULAR AND MOLECULAR WEIGHT DISTRIBUTION OF WATER-INSOLUBLE POLYSACCHARIDES FROM GANODERMA LUCIDUM / Chapter 4.1 --- Introduction --- p.47 / Chapter 4.2 --- Experimental --- p.48 / Chapter 4.2.1 --- Extraction of water-insoluble polysaccharides from Ganoderma Lucidum --- p.48 / Chapter 4.2.2 --- Gel permeation chromatography of water-insoluble polysaccharides --- p.49 / Chapter 4.2.3 --- Phenol sulfuric acid assay --- p.51 / Chapter 4.2.4 --- MALDI-TOF analysis --- p.51 / Chapter 4.2.5 --- Bradford assay --- p.52 / Chapter 4.3 --- Results and discussion --- p.52 / Chapter 4.3.1 --- Extraction of water-insoluble polysaccharides from Ganoderma Lucidum --- p.52 / Chapter 4.3.2 --- Fractionation of water-insoluble polysaccharides using gel permeation chromatography (GPC) --- p.54 / Chapter 4.3.3 --- MALDI-TOF analysis of fractionated polysaccharides --- p.57 / Chapter 4.3.4 --- Carbohydrate content analysis of fractionated polysaccharides --- p.62 / Chapter 4.3.5 --- MW and MWD of water-insoluble polysaccharides extracted from Ganoderma Lucidum --- p.69 / Chapter 4.4 --- Conclusion --- p.71 / Chapter CHAPTER FIVE --- CONCLUDING REMARKS / Chapter 5.1 --- Conclusion --- p.73 / REFERENCES --- p.76 / APPENDIX --- p.81 / Appendix A Reaction scheme of carbohydrate with phenol in phenol-sulfuric acid assay --- p.81
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324686 |
| Date | January 2004 |
| Contributors | Sun, Baizhong., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 80 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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