The genus Bordetella contains several pathogenic species generally
associated with upper respiratory tract infections in warm-blooded animals.
Bordetella pertussis is the etiologic agent of whooping cough. Whooping
cough is presently one of the ten most common causes of death from
infectious diseases and reported by the World Health Organisation (WHO) to
cause 50 million cases and 350000 deaths worldwide per year, mainly among
unvaccinated individuals in poor countries.
The term proteome, in analogy to the term genome, was coined to describe
the complete set of proteins that an organism has produced under a defined
set of conditions. Proteomics has been used to identify novel bacterial
vaccine candidates against several human pathogens. Fueled by growing
DNA sequence information, the analysis of the proteome becomes a valuable
and useful tool for antigen discovery. Much of information about
immunogenic component can be derived from proteomics coupled to
Western blotting, namely immunoproteomics.
v
In the present study, we report first immunoproteomics analysis to identify
candidate antigens of B. pertussis for vaccine development. Different sera
from mice, which were immunized or challenged with B. pertussis, were
analyzed for reactivity by Western blot against whole cell extracts of B.
pertussis Tohama and Saadet strains separated by 2-DE.
We identified 15 immunogenic proteins of Bordetella pertussis as a total (60
kDa chaperonin, heat shock protein, serum resistance protein, putative
substrate-CoA ligase, ATP-dependent protease, preprotein translocase secA
subunit, S-adenosylmethionine synthetase, elongation factor Tu, RNA
polymerase alpha subunit, ketol-acid reductoisomerase, pertactin, lysyl-tRNA
synthetase, serum resistance protein, carbamoyl-phosphate synthase large
chain, 30S ribosomal protein S1 subunit), 6 of which being identified as
immunogenic in a pathogenic microbe (ATP-dependent protease, carbamoylphosphate
synthase large chain, lysyl-tRNA synthetase, putative chromosome
partition protein, preprotein translocase secA subunit, 30S ribosomal protein
S1 subunit) and 5 identified as immunogenic for Bordetella pertussis (RNA
polymerase alpha subunit, S-adenosylmethionine synthatase, putative
substrate-CoA ligase, elongation factor Tu, ketol-acid reductoisomerase) for
the first time.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12608320/index.pdf |
| Date | 01 April 2007 |
| Creators | Altindis, Emrah |
| Contributors | Ozcengiz, Gulay |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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